Your partner in oncology

Contact

OTD
Oncology Therapeutic Development

100 rue Martre
92110 Clichy - FRANCE
Tel:  +33 1 47 15 01 01
Fax: +33 1 45 19 06 59

Contact Us

Techniques

In vitro models and biomarker analysis

  • ATCC oncology solid tumor and hematologic cell lines (panel of over 60 lines)
  • In-house characterized models of paired epithelial/mesenchymal, drug-resistant/sensitive cell lines
  • Analyses of blood/sera, frozen biopsies, paraffin-embedded tissue

 

In vivo models

  • Murine subcutaneous xenograft and orthotopic models, housed in accredited facilities: first and second-line treatments after in vivo drug resistance acquisition
  • MM xenografts
  • Intraperitoneal, intravenous, subcutaneous, oral gavage drug administration

 

Techniques

  • Cell proliferation and viability assays (MTT, LDH, clonogenic, ATPlite, xCELLigence, etc.)
  • Cell cycle and apoptosis assays (flow cytometry)
  • Cell migration and invasion (Matrigel™, Boyden Chamber, Gap Closure)
  • Intracellular signaling pathways exploration/proof of concept evaluations (cell transfections, RNA interference technology)
  • Cell line authentication and mycoplasma testing
  • Drug combination evaluation (Chou-Talalay)
  • Gene expression profile/microarray studies (humanHT-12 v4 expression BeadChip)
  • RNA expression (PCR, quantitative RT-PCR)
  • Protein analyses (Western blotting, ELISA)
  • Immunohistochemistry, immunofluorescence and FISH
  • Pharmacokinetic parameter determinations