Techniques

In vitro models and biomarker analysis

• ATCC oncology solid tumor and hematologic cell lines (panel of over 60 lines)
• In-house characterized models of paired epithelial/mesenchymal, drug-resistant/sensitive cell lines
• Analyses of blood/sera, frozen biopsies, paraffin-embedded tissue

In vivo models

• Murine subcutaneous xenograft and orthotopic models, housed in accredited facilities: first and second-line treatments after in vivo drug resistance acquisition
• MM xenografts
• Intraperitoneal, intravenous, subcutaneous, oral gavage drug administration

Techniques

• Cell proliferation and viability assays (MTT, LDH, clonogenic, ATPlite, xCELLigence, etc.)
• Cell cycle and apoptosis assays (flow cytometry)
• Cell migration and invasion (Matrigel™, Boyden Chamber, Gap Closure)
• Intracellular signaling pathways exploration/proof of concept evaluations (cell transfections, RNA interference technology)
• Cell line authentication and mycoplasma testing
• Drug combination evaluation (Chou-Talalay)
• Gene expression profile/microarray studies (humanHT-12 v4 expression BeadChip)
• RNA expression (PCR, quantitative RT-PCR)
• Protein analyses (Western blotting, ELISA)
• Immunohistochemistry, immunofluorescence and FISH
• Pharmacokinetic parameter determinations